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KMID : 1094720160210050634
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Biotechnology and Bioprocess Engineering
2016 Volume.21 No. 5 p.634 ~ p.640
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Isomerase activity of Candida rugosa lipase in the optimized conversion of racemic ibuprofen to (S)-ibuprofen
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Mortazavi Saideh S.
Chavez-Flores David
Salvador James M.
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Abstract
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The Candida rugosa lipase catalyzed Dynamic Kinetic Resolution of racemic ibuprofen methyl ester produced (S)-ibuprofen in over 90% yield within 72 h at pH 7.6. The best concentration of various buffers for these reactions ranged from 0.2 to 0.5 M. The commercial lipase was found to be acidic altering the final pH of the reaction mixtures. Dimethylformamide co-solvent maintained the reaction pH better than dimethylsulfoxide. Lower concentrations of ibuprofen methyl ester and higher stirring rates led to faster conversions. The minimal amount of lipase needed was 20 mg/mL buffer. Reaction of (R)-ibuprofen methyl ester under the optimized conditions excluding the lipase led to no racemization, indicating that the conversion of (R)-ibuprofen methyl ester to (S)-ibuprofen is catalyzed by the enzyme, thus, indicating Candida rugosa lipase possess Isomerase activity.
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KEYWORD
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dynamic kinetic resolution, Candida rugosa lipase, ibuprofen methyl ester, (S)-Ibuprofen, racemization, dimethylformamide
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